Here are the tropes I had to think of based off my web series:
Animesque - The artstyle of the show is a mix of a child's drawing artstyle and anime.
Aloof Dark-Haired Girl - Gabriella, the tanks team cheerleader might be cute, but she is very serious. You might think she's mean, but she's very nice actually.
Everyone Loves Blondes - Lil' Miss Cheerleader is very cute with her natural blonde hair
Girlish Pigtails - Lil' Miss Cheerleader wears pigtails all the time (Gabriella sometimes wears pigtails only when she's cheerleading, but mostly keeps her hair down)
Girly Girl - Lil' Miss Cheerleader is one
I'm Melting! - In the Halloween special part 2, Lil' Miss Cheerleader gets dumped water by a Random guy who thought she was literally a witch, but it was just a witch costume, making this a failed version of a recreation scene from The Wizard of Oz
Innocent Blue eyes - Lil' Miss Cheerleader's eyes are like diamonds with her blue eyes
Japanese Pop Music - In one art from the official series, Lil' Miss Cheerleader is doing a parody version of Yuko Ogura's Vitamin Love.
Mega Twintails - Lil' Miss Cheerleader has poofy pigtails
Not in Kansas Anymore - Lil' Miss Cheerleader's original hometown is Kansas City, until in the Thanksgiving episode, she stated that she moved from Kansas City to Newgrounds York City, making another Wizard of Oz reference.
Special Guest - on the red carpet animation for Pico Day, Pico from Pico's School is in this, but we don't know if there will be another one.
That's all the tropes I know, LOL